Cell cultures in space spontaneously grow in three dimensions (3D), which results in characteristics more representative of how cells grow and function in living organisms. But in microgravity, routine manipulation of cell cultures is challenging. Magnetic 3D Cell Culture for Biological Research in Microgravity (Magnetic 3D Cell Culturing) uses magnetized cells and tools to make it easier to handle cells and cultures, and to improve the reproducibility of experiments. This approach also makes it possible to generate two-dimensional (2D) cultures as controls, and to determine whether biological events in these monolayer cultures result from gravity or substrate attachment